Revisión: Métodos para la cuantificación de la proteína Β-Lactoglobulina (β-LG)

Yamile Jiménez-Alfonso; Claudia Constanza  Pérez Rubiano; Roy José Andrade-Becerra

doi:10.33571/rpolitec.v20n40a3

Authors

Yamile Jiménez-Alfonso Universidad Pedagógica y Tecnológica de Colombia https://orcid.org/0000-0001-9902-1466
Claudia Constanza Pérez Rubiano docente https://orcid.org/0000-0003-0698-1826
Roy José Andrade-Becerra docente https://orcid.org/0000-0002-9681-2067

DOI:

https://doi.org/10.33571/rpolitec.v20n40a3

Keywords:

Globulins, β-lactoglobulin, Milk proteins, Whey proteins, Analytical techniques

Abstract

The β-lactoglobulin (β-LG) is the protein found in the highest proportion in whey and represents approximately 50% of the total whey protein and 10% of the total milk protein. The β-LG is characterized by being a globular protein of the lipocalin family, its main function is to transport hydrophobic molecules, in addition, it is widely studied for its various techno-functional properties, mainly for its high value as a food ingredient. However, since it is not present in the composition of human milk, it is identified as an allergen, which can represent a significant risk to the health of infants. Therefore, several methods have been developed in recent years, including spectrophotometric, electrophoretic, immunoassay and chromatographic methods for the separation and quantification of β-LG in whey. Because the quantification of this protein is fundamental for milk processing, detection of adulterated dairy products and generation of new contributions in the field of research, it is important to expand knowledge regarding its fraction and the development of new dairy products with specific properties. In this review, the different methodologies and protocols used in the quantification of β-LG are compiled. In addition, fundamentals, advantages and limitations of each one of them are discussed, as well as their future projection, in order to broaden the vision regarding the quantification of whey proteins, specifically β-LG.

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Author Biographies

Claudia Constanza Pérez Rubiano, docente

Docente e investigadora de la Universidad Pedagógica y Tecnológica de Colombia. Bióloga con Maestría en microbiología, actualmente cursa el doctorado en Ciencias Biológicas y ambientales, con varios años de experiencia en la enseñanza y la investigación en microbiología ambiental y de alimentos.

Roy José Andrade-Becerra, docente

Docente e investigador de la Universidad Pedagógica y Tecnológica de Colombia. Médico Veterinario, Especialista en Docencia Universitaria, Magister en Microbiología y PhD. En Ciencias Agrarias. Director del grupo de investigación en sanidad y producción animal del trópico alto colombiano GIPATRACOL.

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