Perfil enzimático digestivo de Leiarius marmoratus (Siluriformes: Pimelodidae) en el medio natural
Autores/as
-
Mariana Catalina Gutiérrez Espinosa
Universidad de los Llanos
https://orcid.org/0000-0001-6127-9955
- Beatriz Elena Zapata Berruecos
DOI:
https://doi.org/10.33571/rpolitec.v15n28a7Palabras clave:
Acuicultura, peces tropicales, nutrición, alimento, hábito alimenticioResumen
El objetivo de esta investigación fue determinar el perfil enzimático digestivo de ejemplares adultos de Leiarius marmoratus, extraídos del medio natural. El perfil enzimático fue caracterizado en ejemplares de yaque con peso y longitud de 1037±164 g y 50±4 cm respectivamente. Una vez capturados los peces fueron trasladados al laboratorio, sacrificados y se extrajo el trato digestivo (estómago e intestino anterior, medio y posterior), este fue congelado en nitrógeno líquido y almacenado a -20°C, hasta los respectivos análisis. A lo largo del tracto digestivo de se detectó hidrólisis de proteína, carbohidratos y lípidos, siendo mayor esta actividad a nivel de intestino anterior. Presentó actividad proteolítica inespecífica ácida (estómago) y alcalina (intestino). Se detecto actividad lipolítica y de amilasa en estómago, intestino anterior y medio. Los análisis muestran actividad enzimática significativa (P <0,05) a nivel del intestino anterior, lo que confirma sus hábitos de tipo omnívoro.
The objective of this investigation was to determine the enzymatic digestive profile of Leiarius marmoratus adults, extracted from the natural environment. The enzymatic profile was characterized in Yaque with weight and length of 1037 ± 164 g and 50 ± 4 cm respectively. Once the fish were captured, they were transferred to the laboratory, slaughtered and the digestive treatment was extracted (stomach and intestine), which was frozen in liquid nitrogen and stored at -20 ° C. Hydrolysis of protein, carbohydrates and lipids was detected along the digestive tract, with this activity being greater at the level of the anterior intestine (34.3, 0.36 y 8.5 U/mg of protein, respectively). It presented non-specific proteolytic activity acid (stomach) and alkaline (intestine). The lipolytic and amylase activity were observed in the stomach, the midgut. The analyzes show significant enzymatic activity (P <0.05) at the level of the anterior intestine, which confirms their omnivorous habits.
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Maldonado-Ocampo, J.A., Vari, R.P. y Usma, J.S. Checklist of the freshwater fishes of Colombia. Biota Colombiana, 9(2), 143-237, 2008.
Acosta-Santos, A., Agudelo-Córdoba, E., Ajíaco-Martínez, R. y Ramírez-Gil, H. Leiarius marmoratus (Gill 1870). Ficha Amazonas. En: Catálogo de recursos pesqueros continentales de Colombia (Eds Lasso, C., Agudelo- Córdoba, E., Ramírez-Gil, H., Morales-Betancourt, M., Ajiaco-Martínez, R., Gutiérrez, F., Usma- Oviedo, J.S., Muñoz-Torres, S. y Sanabria-Ochoa, A.), Instituto de Investigaciones de Recursos Biológicos Alexander von Humboldt. 448-45, 2011.
Ramírez-Gil, H. y Ajíaco-Martínez, R. Leiarius marmoratus (Gill 1870). Ficha Orinoco. En: Catálogo de recursos pesqueros continentales de Colombia (Eds Lasso, C., Agudelo- Córdoba, E., Ramírez-Gil, H., Morales-Betancourt, M., Ajiaco-Martínez, R., Gutiérrez, F., Usma- Oviedo, J.S., Muñoz-Torres, S. & Sanabria-Ochoa, A.), Instituto de Investigaciones de Recursos Biológicos Alexander von Humboldt, 451-454, 2011
Layman, C.A., Winemiller, K.O., Arrington, D.A., y Jepsen, D.B. Body size and trophic position in a diverse tropical food web. Ecology, 86 (9): 2530-2535, 2005.
Cruz-Casallas, N.E., Medina-Robles, V.M., Cruz-Casallas, P.E. y Suárez-Mahecha, H. Nutritional characterisation of filetes of bagre yaque (Leiarius marmoratus) pertinent of natural extraction and crop. Orinoquia, 16: 259-259, 2012.
Kossowski, C. Observaciones preliminares sobre reproducción inducida y ontogénesis temprana del bagre negro, Leiarius marmoratus (Piscis, Siluriformes). Memorias XXVI Convención Anual AsoVAC. AsoVAC- Universidad de Carabobo. Valencia (Edo. Carabobo), Venezuela. 232, noviembre 1986.
Escobar, L., y Mojica, H.O. Ensayos preliminares de reproducción inducida del yaque, Leiarius marmoratus (Gill, 1870) (Pisces: Siluriformes:Pimelodiade) en la Orinoquía colombiana. Bol. Cien. INPA, 5, 9-26, 1997.
Marciales-Caro, L.J., Cruz-Casallas, N.E., Díaz-Olarte, J.J., Medina-Robles, V. M., y Cruz-Casallas, P.E. Crecimiento y sobrevivencia de post-larvas de bagre rayado (Pseudoplatystoma sp.) y yaque (Leiarius marmoratus) consumiendo una dieta seca. Revista Colombiana de Ciencias Pecuarias, 24(2), 179-190, 2011
Ramírez-Merlano, J.A., Otero-Paternina, A.M., Corredor-Santamaría, W., Medina-Robles, V.M., Cruz-Casallas, P.E., y Velasco-Santamaría, Y.M. Utilización de organismos vivos como primera alimentación de larvas de yaque (Leiarius marmoratus) bajo condiciones de laboratorio. Revista Orinoquia, 14(1), 45-58, 2010.
Sánchez, J. A. M., Moyetones, F. y Cerdá, M.J. Influencia del contenido proteico en el crecimiento de alevines de bagre yaque, Leiarius marmoratus, alimentados con concentrados comerciales. Zootecnia Tropic, 27, 187-194, 2009.
Gupta, S.S. y Homechoudhury, S. Comparison of trophic niche and digestive enzymes of four species of catfishes of the Punarbhaba River in India. Indian Journal of Fisheries, 58 (4), 79-85, 2011.
López-Vásquez, C.A. y Castro-Pérez, A.L. Digestive enzymes of eight Amazonian teleosts with different feeding habits. Journal of Fish Biology, 74, 1620-1628, 2009.
Fu, X., Xue, C., Miao, B., Li, Z., Gao, X., y Hirata, T. Distribution and seasonal activity variation of proteases in digestive tract of sea cucumber Stichopus japonicus. Fisheries Science, 72, 1130-1132, 2006.
Braga, L. Primeros registros para la Argentina de tres especies de la familia Rivulidae (Teleostei, Cyprinodontiformes). Physis, B, Buenos Aires, Argentina, 57(132-133), 11-15, 1999.
Hidalgo, M.C., Urea, E. y Sanz, A. Comparative study of digestive enzymes in fish with different nutritional habits. Proteolytic and amylase activities. Aquaculture, 170, 267-283, 1999.
Albro, P.W., Hall, R.D., Corbett, J.T. y Schroeder, J. Activation of nonspecific lipase (EC 3.1. 1.-) by bile salts. Biochimica et Biophysica Acta (BBA)-Lipids and Lipid Metabolism 835(3), 477-490, 1985.
Lundstedt, L.M., Melo, J. y Moraes, G. Digestive enzymes and metabolic profile of Pseudoplatystoma corruscans (Teleostei: Siluriformes) in response to diet composition. Comparative Biochemistry and Physiology Part B, 137, 331–339, 2004.
Bradford, M.M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72: 248-254, 1976.
González-Zamorano, M., Navarrete Del Toro, M. A. y García-Carreño, F. L. Exogenous proteinases as feed supplement for shrimp: in vitro evaluation. Aquaculture Nutrition, 19, 731-740, 2013.
Hakim, Y., Harpaz, S. y Uni, Z. Expression of brush border enzymes and transporters in the intestine of European sea bass (Dicentrarchus labrax) following food deprivation. Aquaculture, 290(1), 110-115, 2009.
Debnath, D., Pa, A.K., Sahu, N.P., Yengkokpam, S., Baruah, K., Choudhury, D. y Venkateshwarlu, G. Digestive enzymes and metabolic profile of Labeo rohita fingerlings fed diets with different crude protein levels. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 146(1),107-114, 2007.
1. El-Sayed, A. F., Nmartinez, I. y Moyano, F.J. Assessment of the effect of plant inhibitors on digestive proteases of nile tilapia using in vitro assays. Aquaculture International, 8(5), 403-415, 2000.
1. German, D.P., Horn, M.H. y Gawlicka, A. Digestive enzyme activities in herbivorous and carnivorous prickleback fishes (Teleostei: Stichaeidae): ontogenetic, dietary, and phylogenetic effects. Physiological and Biochemical Zoology, 77(5), 789-804, 2004.
De Almeida, L.C., Lundstedt, L.M. y Moraes, G. Digestive enzyme responses of tambaqui (Colossoma macropomum) fed on different levels of protein and lipid. Aquaculture Nutrition, 12(6), 443-450, 2006.
Hernandez-Sámano, A.C., Guzman-García, X., Garcia-Barrientos, R. y Guerrero-Legarreta, I. Actividad enzimática de proteasas de Cyprinus carpio (Cypriniformes: Cyprinidae) extraídas de una laguna contaminada en México. Rev. Biol. Trop., 65 (2), 589-597, 2017.
Hakim, Y., Uni, Z., Hulata, G. y Harpaz, S. Relationship between intestinal brush border enzymatic activity and growth rate in tilapias fed diets containing 30% or 48% protein. Aquaculture 257(1), 420-428, 2006.
Moraes, G. y Bidinotto, P.M. Induced changes in the amylohrydrolytic profile of the gut of Piaractus mesopotamicus (Holmberg, 1885) fed different levels of soluble carbohydrate: its correlation with metabolic aspects. Revista de ictiologia, 8, 47-51, 2000.
Tengjaroenkul, B., Smith, B. J., Caceci, T. y Smith, S. A. Distribution of intestinal enzyme activities along the intestinal tract of cultured Nile tilapia, Oreochromis niloticus L. Aquaculture, 182(3), 317-327, 2000.
Liu, Z.Y., Wang, Z. y Zhang, J. An acidic protease from the grass carp intestine (Ctenopharyngodon idellus). Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 149(1), 83-90, 2008.
Natalia, Y., Hashim, R., Ali, A. y Chong, A. Characterization of digestive enzymes in a carnivorous ornamental fish, the Asian bony tongue Scleropages formosus (Osteoglossidae). Aquaculture, 233(1), 305-320, 2004.
Chong, A.S., Hashim, R., Chow-Yang, L., y Ali, A.B. Partial characterization and activities of proteases from the digestive tract of discus fish (Symphysodon aequifasciata). Aquaculture, 203(3), 321-333, 2002.
De Almeida, L. D. Desempenho produtivo, eficiência digestiva e perfil metabólico de juvenis de tambaqui, Colossoma macropomum (Cuvier, 1808) alimentados com diferentes taxas carboidrato/lipídio [Teses doutorado] Universidade Federal de São Carlos. São Carlos-SP, Brasil, 2010.
Eroldoğan, O.T., Suzer, C., Taşbozan, O. y Tabakoğlu, S. The effects of rate-restricted feeding regimes in cycles on digestive enzymes of gilthead sea-bream, Sparus aurata. Turkish Journal of Fisheries and Aquatic Sciences, 8(1), 49-54, 2008.
Pérez-Jiménez, A., Cardenete, G., Morales, A. E., García-Alcázar, A., Abellán, E. y Hidalgo, M.C. Digestive enzymatic profile of Dentex dentex and response to different dietary formulations. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 154(1), 157-164, 2009.
Lovell, T. Nutrition and feeding of fish. New YorK, NY: Van Nostrand Reinhold, 1988.
Yamakami, R. Efeitos de carboidratos no desempenho, na digestao e no metabolismo de matrinxa (Brycon amazonicus) e hibridos de pintado (Pseudoplatystoma corruscans x Pseudoplatystoma fasciatum) em um periodo curto de alimentacao.: [Teses doutorado] Universidade Federal de São Carlos. São Carlos-SP. 2012.
Sunde, J. Digestive protease activities, growth and feed utilisation in Atlantic salmon (Salmo salar L.): Tesis (Doctor Scientiarium) - Institute of Marine Research (IMR)-Department of Aquaculture, University of Bergen, Bergen, Norway, 2006.
Kuz'mina, V.V. Influence of age on digestive enzyme activity in some freshwater teleosts. Aquaculture 148(1), 25-37, 1996.
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