Análisis in silico de una proteína similar a phag en Ralstonia Eutropha H16 potencialmente involucrada en la síntesis de polihidroxialcanoatos

Melissa Uribe Acosta, Andrés Felipe Villa Restrepo

Resumen


Los polihidroxialcanoatos (PHA) son sintetizados por las bacterias como material de reserva de carbono. La proteína PhaG dirige el carbono proveniente de fuentes de carbono no relacionadas como el glicerol, que son metabolizados a través de la síntesis de ácidos grasos de novo (FAS), hacia la síntesis de PHA. El gen que codifica esta proteína no ha sido aún encontrado en el genoma de Ralstonia eutropha H16, un organismo modelo. A través de la comparación con proteínas PhaG ya conocidas, una proteína similar a PhaG, fue encontrada siendo codificada por el gen H16_A0147 y la presencia del gen confirmada preliminarmente utilizando PCR. Este es el primer estudio que muestra la presencia y características de una proteína similar a PhaG en R. eutropha H16 y representa el primer paso en la identificación de una conexión entre las rutas metabólicas FAS y de PHA en esta bacteria modelo. Estudios de bloqueo de genes y actividad enzimática son necesarios para confirmar esta relación potencial que podría mejorar la producción industrial de PHA y la utilización de residuos agroindustriales como el glicerol.


Palabras clave


Polyhydroxyalkanoates, Ralstonia eutropha H16, non-related carbon sources.

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DOI: https://doi.org/10.33571/rpolitec.v15n29a5

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Revista Politécnica 
ISSN: 1900-2351 
ISSN: 2256-5353 (En línea)
DOI:  10.33571/rpolitec