REVISTA POLITÉCNICA, Vol. 15, Núm. 29

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ANÁLISIS IN SILICO DE UNA PROTEÍNA SIMILAR A PHAG EN RALSTONIA EUTROPHA H16 POTENCIALMENTE INVOLUCRADA EN LA SÍNTESIS DE POLIHIDROXIALCANOATOSD

Uribe Acosta Melissa, Villa Restrepo Andrés Felipe

Resumen

Polyhydroxyalkanoates (PHA) are synthesised by bacteria as carbon storage material. The protein PhaG directs carbon from non-related carbon sources such as glycerol, metabolised through fatty acid de novo synthesis (FAS) pathway, with PHA synthesis. The gene that codifies for this protein has not yet been found in the genome of Ralstonia eutropha H16, a model organism. By bioinformatic comparison to already known PhaG proteins, a PhaG-like protein was found codified by gene H16_A0147 and presence of the gene was preliminary confirmed by PCR. This is the first study that shows the presence and characteristics of a PhaG-like protein in R. eutropha H16 and represents the first step for the identification of a connection between FAS and PHA pathways in this model bacterium. Further gene deletion and enzymatic activity studies are necessary to confirm this potential relationship, which could improve industrial PHA production and utilisation of agro-industrial residues such as glycerol.

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10.33571/rpolitec.v15n29a5

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Revista Politécnica 
ISSN: 1900-2351 
DOI:  10.33571/rpolitec