Validation and implementation of a methodology for the determination of total organic carbon in soils and sediments according to ISO 14235:1998
Keywords:
Total organic carbon, validation, marine sediment, lake sediment, soilAbstract
Total organic carbon (TOC) quantification in sediments and soils samples is a tool to evaluate the organic matter content in these matrixes. This study aims to verify the fitness for use of the methodology for spectrophotometric analysis by sulphochromic oxidation described in ISO 14235: 1998 with adjustments related to digestion conditions, and its applicability to the analysis of samples of such matrices in the GAIA Research Group Laboratory of University of Antioquia. The validation was performed by determining the following parameters: linearity, working range, detection and quantification limits, accuracy and precision (repeatability and intermediate precision). Results show a significant matrix effect (α= 0.05) for soil samples analysis, the measurement range was established between 2.0 – 10 mg C, with a detection limit of 0.4 mg C and good linear fitness ((r2 > 0.995). Coefficients of variation ranged between the expected values for the concentration levels tested (<5%). The recoveries obtained using certified reference material (SRM-1944) were acceptable (102-109%). This leads to conclude that the studied methodology can be used for analysis of TOC in soil and sediment reliably.Article Metrics
|Abstract: 908 | PDF (Español (España)): 1037 |References
M. W. I. Schmidt, A. G. Noack, and G. Osmond, “Analysis, distribution, implications, and current challenges,” vol. 14, no. 3, pp. 777–793, 2000.
M. Schnitzer, “Soil organic matter: the next 75 years,” Soil Sci., vol. 151, no. 1, pp. 41–58, 1991.
R. D. Harter and R. Naidu, “Role of Metal-Organic complexation in metal sorption by Soils,” Adv. Agron., vol. 55, no. C, pp. 219–263, 1995.
B. a Schumacher, “Methods for the Determination of Total Organic Carbon in Soils and Sediments,” Carbon N. Y., vol. 32, no. April, p. 25, 2002.
I. S. O. ISO, ISO 14235: Soil quality- Determination of organic carbon by sulfochromic oxidation, vol. 1. 1998.
ISO, “ISO/IEC 17025 General requirements for the competence of testing and calibration laboratories,” International Standard. pp. 1–36, 2005.
Citac and Eurachem, “Quantifying Uncertainty in Analytical Measurement,” English, vol. 3rd, p. 133, 2012.
S. Method, P. Requirements, and A. Nutritionals, “Appendix F: Guidelines for Standard Method Performance Requirements,” 2012.
J. H. McKenna and P. H. Doering, “Measurement of dissolved organic carbon by wet chemical oxidation with persulfate: Influence of chloride concentration and reagent volume,” Mar. Chem., vol. 48, no. 2, pp. 109–114, 1995.
H. E. Gaudette, W. R. Flight, L. Toner, and D. W. Folger, “An inexpensive titration method for the determination of organic carbon in recent sediments.,” J. Sediment. Petrol., vol. 44, no. 1, pp. 249–253, 1974.
J. W. T. Tung and P. a Tanner, “Instrumental determination of organic carbon in marine sediments,” Mar. Chem., vol. 80, no. 2–3, pp. 161–170, 2003.
AEFI: Asociación Española de Farmaceúticos de la Indutria, “Validación de métodos analíticos,” Barcelona, 2001.
P. King, H. Kennedy, P. P. Newton, T. D. Jickells, T. Brand, S. Calvert, G. Cauwet, H. Etcheber, B. Head, A. Khripounoff, B. Manighetti, and J. Carlos Miquel, “Analysis of total and organic carbon and total nitrogen in settling oceanic particles and a marine sediment: An interlaboratory comparison,” Mar. Chem., vol. 60, no. 3–4, pp. 203–216, 1998.
I. Bisutti, I. Hilke, and M. Raessler, “Determination of total organic carbon – an overview of current methods,” TrAC Trends Anal. Chem., vol. 23, no. 10–11, pp. 716–726, Nov. 2004.
_
