Red inalámbrica de biosensores enzimáticos para la detección de contaminantes en aguas

Duvan Fernando Morales Castaño, Juan Bernardo Cano Quintero, Nelson De Jesus Londoño Ospina


En este artículo se presenta el desarrollo de una red inalámbrica de biosensores amperimétricos, que podrá ser distribuida en las fuentes de agua potable, con el fin de alertar sobre la presencia de contaminantes tóxicos. Este sistema no reemplazará los métodos tradicionales para la detección de contaminantes, pero tiene la ventaja de ser un sistema de bajo costo, ideal para aplicaciones en campo y donde sea necesario el procesamiento de una gran cantidad de muestras. Se presentan resultados del desarrollo de los circuitos electrónicos necesarios para la transducción amperométrica, los circuitos para el procesamiento de datos y la comunicación inalámbrica.

This article presents the development of an amperometric biosensor wireless network, which can be distributed in drinking water sources, in order to warn about toxic contaminants presence. This system will not replace traditional contaminants detection methods, but it has the advantage of being a low cost system, ideal for field applications and where the processing of a large number of samples is necessary. it can be concluded that the biosensors do not have a linear response to the analyte concentration, so their use is qualitative and not quantitative. The results of the development of the electronic circuits for the amperometric transduction, the circuits for data processing and wireless communication are presented.

Palabras clave

Biosensor;Amperometría;Red inalámbrica;Sensores

Texto completo:



G.Pezzotti, A.Tibuzzi, T.Lavecchia, G.Rea, A.Serafini, M.T.Giardi. Portable instruments based in multiband fluorescence transducers for biosensing applications. Revista Politecnica, Year 3, n° 5, pag 123- 128 Medellin, December 2007 ISSN 1900-2351.

K. Buonasera, A. Gabrielli, G. Pezzotti Escobar, R. Briancesco, S. Paduano, M. Semproni, L. Bonadonna. Rapid detection of Escherichia coli in marine water samples by remote optical biosensor system. The 12th Workshop on Biosensors & Bio-Analytical Microtechniques in Environmental, Food & Clinical Analysis, ISBN 9788880802457, September 2017, Rome, Italy.

G.Pezzotti, K . Buonasera, I. F. Pezzotti, J.B. Cano. Device and method for detecting biological contaminants, in particular coliform bacteria in water samples. PCT.IB2016.055263 WIPO, September 2016.

K. Buonasera, G. Pezzotti Escobar, A. Gabrielli, A. Nicolosi, R. Briancesco, S. Paduano, M. Semproni, L. Bonadonna. A Completely Automatic Optical Biosensor System for Stressed Bacteria In Marine Water. Book XV IHSS Pharmacological Research And Analytical Approaches, ISBN 9788867416592, Pisa University Press, October, 2016, Italy.

K. Buonasera, J. Griffiths, D. Hernández, T. Noguer, T. Ziemann, G. Pezzotti. A new biosensor for the screening of toxic compounds in water samples. 2nd International Conference on Bio-sensing Technology, October 10-12, 2011, Amsterdam, The Netherlands

M. Turemis, S. Silletti, G. Pezzotti, J. Sanchís, M. Farré, M T. Giardi, L. Moro. Optical biosensor based on the microalga-paramecium symbiosis for improved marine monitoring. Sensors and Actuators B: Chemical (ISSN 0925-4005). Elsevier Science. April 2018.

L. Moro, G. Pezzotti; M. Turemis; J. Sanchís; M. Farré; R. Denaro; M. G. Giacobbe; F. Crisafi; M T. Giardi. Fast Pesticide Pre-Screening in Marine Environment Using a Green Microalgae-based Optical Bioassay. Marine Pollution Bulletin 129 (2018) 212–221 Elsevier Science February 2018, (ISSN 0025-326X). DOI: 10.1016/j.marpolbul.2018.02.036.

M.T.Giardi, M.Lambreva, G.Rea, A.Antonacci, A.Serafini, G.Pezzotti, T.Lavecchia. Computational and biotechnological approaches for the construction of enviromental biosensors for herbicide analyses. Environmental and Health Biosensor, Roma, 9-10 October 2007, ISPESL.

J.Cano, K.Buonasera, G.Pezzotti, M.T.Giardi. New platform of biosensors based on fluorescence enviromental applications. Revista Politécnica, Politécnico Colombiano JIC. ISSN 1900-2351, P 124-132, year 3, N°13 2011, Medellín.

K.Buonasera, G.Pezzotti, I.Pezzotti, J.Cano, MTGiardi. Biosensors: new frontiers for the environmental analysis. Revista Politecnica, Politecnico Colombiano JIC. ISSN 1900-2351, P 93-100, year 3, N°13 2011, Medellín.

K.Buonasera, I.Pezzotti, G.Rea, G.Pezzotti, M.T.Giardi - “Design of a portable instrument for environmental applications by amperometric measurements on biological material” Revista Politecnica, Politecnico Colombiano JIC. Year 5, n° 9, pag 107- 114 Medellín, December 2009 ISSN 1900-2351.

M.T.Giardi, V.Scognamiglio, G.Rea, G. Rodio, A.Antonacci, M.Lambreva, A.Tibuzzi, G.Pezzotti, U.Johanningmeier. Optical biosensors for environmental monitoring based on computational and biotechnological tools for engineering the photosynthetic D1 protein of Chlamydomonas reinhardtii. Biosensor and Bioelectronics, Volume 25 issue 2, pages 294-30, Elsevier, 15 October 2009; (ISSN): 0956-5663, DOI: 10.1016/j.bios.2009.07.003.

J. Cano, D. Giannini, G. Pezzotti, G. Rea, M.T. Giardi. Space Impact and Technological Transfer of a Biosensor Facility to Earth Application for Environmental Monitoring. Recent Patents on Space Technology, 2011, Volume 1, No. 1, March 15, 2011; ISSN 2210-6871; Bentham Science Publishers.

G.Pezzotti, M.T.Giardi, M.Lambreva, G.Rea, I.Pezzotti. Development of new environmental biosensors instruments for herbicides analyses. Revista Politecnica, Year 4, n° 6, pp. 116- 123 Medellin, January 2008 ISSN 1900-2351.

J.B. Cano Quintero, K. Buonasera, G. Pezzotti, M.T. Giardi. Multichannel fluorimeter for biosensor applications on environmental field. pag 79-83, Lecture Notes in Electrical Engineering,Sensors , Springer New York 2013, ISSN 1876-1100,1876-1119 doi: 10.1007/978-1-4614-3860-1_12.

M.T.Giardi, M.Lambreva, G.Rea, A.Antonacci, A.Serafini, G.Pezzotti, T.Lavecchia. Computational and biotechnological approaches for the construction of enviromental biosensors for herbicide analyses. Enviromental and Health Biosensor, 10th October 2007, ISPESL, Rome, Italy.

V.Scognamiglio, G.Pezzotti, I.Pezzotti, J.Cano, K.Buonasera, D.Giannini, M.T.Giardi. Biosensors for Effective Environmental and Agrofood Protection and Commercialization: from research to market. Microchimica Acta, SpringerLink ISSN 0026-3672, Jan 2010., DOI: 10.1007/s00604-010-0313-5.

R. Penu, S. C. Litescu, S. A.V. Eremia, G.L. Radu, M.T. Giardi, G. Pezzotti, G. Rea. Application of an optimized electrochemical sensor in astaxanthin antioxidant properties monitoring against lipoperoxidation, during algae accumulation. New Journal of Chemistry - The Royal Society of Chemistry 2013, (ISSN): 1144-0546, DOI: 10.1039/C5NJ00457H.

A.Tibuzzi, G.Pezzotti, G.Rea, D.Bianconi, M.T.Giardi. A RC-based biosystem for measuring anti-oxidizing power. Technical Proceedings of the 2007 NSTI Nanotechnology Conference and Trade Show, Volume 2, Chapter 6: Biosensors, pp. 519 – 522, ISBN:1-4200-6183-6.

V.Scognamiglio, G.Pezzotti, I.Pezzotti, J.Cano, I.Manfredonia, K.Buonasera, F. Arduini, D. Moscone, G. Palleschi, M.T.Giardi. Towars an integrated Biosensors array for simultaneous and rapid multi-analisys of endocrine disrupting chemicals. Analytica Chimica Acta, Elsevier Science, 2012, (ISSN): 0003-2670, DOI: 10.1016/j.aca.2012.09.010.

G.Pezzotti, J.Cano, K.Buonasera. Biosensing technologies for space applications. Revista Politecnica, Politecnico Colombian JIC. ISSN 1900-2351, P 133-143, year 3, N°13 2011, Medellin, Colombia.

M.Turemis, M.T.Giardi, G.Pezzotti. Space impact of a biosensor facility for water control in ISS and technological transfer to Earth applications. workshops “Bioregenerative Life Support” of International Space Life Sciences Working Group- ISLSWG, Turin, 18-19 May 2015.

V.Scognamiglio, D.Raffi, M.Lambreva, G.Rea, A.Tibuzzi, G.Pezzotti, U. Johanningmeier, M.T.Giardi. Chlamydomonas reinhardtii genetic variants as probes for fluorescence sensing system in detection of pollutants. Analytical and Bioanalytical Chemistry, Springer Berlin / Heidelberg ISSN1618-2642 394 (4), p.1081-1087, Jun 2009.

I.Manfredonia, M.Barbieri, R.Verzicco, C.Stallo, M.Ruggieri, T.Lavecchia, M.Turemis, G.Pezzotti - “Performance Analysis for Steady Flow Generation and Improved Readout Signal in Amperometric Biosensors”, IEEE Sensors Journal Sensors - 11214-2014, 2015 (ISSN 1530-437X)

A.Tibuzzi, G.Pezzotti, T.Lavecchia, G.Rea, M.T.Giardi. A Portable Light-Excitation Equipped Bio-Amperometer for Electrogenic Biomaterials to Support the Technical Development of Most Biosensors. Sensors & Transducers Journal, Volume 88, Issue 2, ISSN 1726-5479 February 2008.

A.Tibuzzi, G.Rea, G.Pezzotti, D.Esposito, U.Johanningmeier, M.T.Giardi. A new miniaturized multiarray biosensor system for fluorescence detection. Journal of Physics: Condensed Matter, vol. 19 Issue 39, 395006, ISSN 0953-8984, 2007.

M.Lambreva, G.Pappalettera, A.Palasciano, P.Boffi, V.Scognamiglio, G.Pezzotti, M.T.Giardi. Experimental Laser-induced fluorescence sensor for remote detection of herbicide action on plants. XIII International Symposium on Luminescence Spectrometry, Analytical luminescence (ISLS 2008), John Wiley & Sons, Vol. 23, Issue 4, pp.191-280, July 2008, Bologna, Italy.

G.Pezzotti, M.T.Giardi, G.Rea, A.Tibuzzi, M.Lambreva. Portable instrument based in biosensoristic systems for applications with biological material. 1st International symposium on instrumentation and industrial control, Memoria, Numero 38, ISBN 16920368, ITM, April 2008, Medellin, Colombia.

G.Pezzotti, A.Tibuzzi, T.Lavecchia, G.Rea, M.T.Giardi. Portable bio-amperometer for photoactive biomaterial monitoring. 13th Italian Conference on Sensors and Microsystems AISEM 2008 - XIII Conferenza Annuale, 19 – 21, February 2008, Rome, Italy ISBN 978-981-283-597-0.

J.B. Cano, K. Buonasera, G. Pezzotti “Transduction methods used on biosensors: amperometry and fluorescence” Rev. Fac. Ing. Univ. Antioquia N. 72, pp. 104-115, 2014 (ISSN 0120-6230).

K.Buonasera, G.Pezzotti, V.Scognamiglio, A.Tibuzzi, M.T.Giardi. New Platform of Biosensors for Prescreening of Pesticide Residues To Support Laboratory Analyses. Journal Agricultural and Food Chemistry. 58, 5982–5990; of American chemical society 2010, (ISSN): 0021-8561.

I. Manfredonia, C. Stallo, M. Ruggieri, K. Buonasera, G. Pezzotti.. Architecture design of an early warning monitoring system for chemical water bodies contamination. Conference: 4th International Conference on Wireless Communications, Vehicular Technology, Information Theory and Aerospace & Electronic Systems (VITAE) Location: Aalborg, Denmark May 2014, ISBN: 978-1-4799-4626-6.

B. Bucur, F.D. Munteanu, J. L. Marty, A. Vasilescu. Advances in enzyme-based biosensors for pesticide detection. Biosensor, 8(2). 2018.

M. Zennaro, I. C. T. P. Trieste-Italy. Introduction to Wireless Sensor Networks. 2012.

R. Hidalgo, J. I. Moreno Routing Design in Wireless Sensor Networts and Solution for Healthcare Environment. IEEE Latin Am. Trans, 9. 2011.

Ali, H., Soe, J. K., & Weller, S. R. A real-time ambient air quality monitoring wireless sensor network for schools in smart cities. In Smart Cities Conference (ISC2), IEEE First International (pp. 1-6). 2015.

Lara, R., Benítez, D., Caamaño, A., Zennaro, M., & Rojo-Álvarez, J. L. On real-time performance evaluation of volcano-monitoring systems with wireless sensor networks. IEEE Sensors Journal, 15(6), 3514-3523. 2015.

Polo, J., Hornero, G., Duijneveld, C., García, A., & Casas, O. Design of a low-cost Wireless Sensor Network with UAV mobile node for agricultural applications. Computers and electronics in agriculture, 119, 19-32. 2015.

Vikram, N., Harish, K. S., Nihaal, M. S., Umesh, R., Shetty, A., & Kumar, A. A low-cost home automation system using wi-fi based wireless sensor network incorporating Internet of Things (IoT). In Advance Computing Conference (IACC), IEEE 7th International (pp. 174-178). 2017.

Raspberry Pi Foundation. Disponible en: [consultado el 15 de noviembre de 2018].

Electrodos serigrafiados Metrohm DropSens, Disponible en: [consultado el 24 de noviembre de 2018].



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